Comparing distributions of experimental and random diffusion values (p-values for KS tests)
Seeds	Recipients	Randomize Seeds (degree-matched)	Randomize Recipients (degree-matched)	Randomize Seeds (uniform)	Randomize Recipients (uniform)
Binary_EA_as1 Exclusive	TIER1_GS	2.00e-11	2.71e-11	1.15e-11	2.01e-12
TIER1_GS Exclusive	Binary_EA_as1	1.85e-01	9.42e-02	1.15e-02	3.51e-02
Binary_EA_as1 Exclusive	TIER1_GS Exclusive	7.91e-10	6.40e-10	8.40e-10	6.40e-10
TIER1_GS Exclusive	Binary_EA_as1 Exclusive	5.81e-01	2.27e-01	1.58e-02	1.13e-01
Binary_EA_as1 Exclusive	Overlap	2.89e-03	8.41e-03	3.53e-03	4.21e-04
TIER1_GS Exclusive	Overlap	9.14e-09	6.40e-08	1.83e-09	1.28e-07
Overlap	Exclusives	1.09e-05	3.43e-08	7.28e-13	2.92e-08



Evaluating how well Binary_EA_as1 genes are linked to TIER1_GS genes, comparing against random

ROC results
Seeds	Recipients	AUROC	Z-score for Random Seeds (degree-matched)	Z-score for Random Recipients (degree-matched)	Z-score for Random Seeds (uniform)	Z-score for Random Recipients (uniform)
Binary_EA_as1 Exclusive	TIER1_GS	0.89	4.73	8.68	4.62	7.29
TIER1_GS Exclusive	Binary_EA_as1	0.57	3.02	3.03	2.49	2.54
Binary_EA_as1 Exclusive	TIER1_GS Exclusive	0.88	4.78	6.45	3.85	6.56
TIER1_GS Exclusive	Binary_EA_as1 Exclusive	0.56	2.60	2.38	1.80	2.44
Binary_EA_as1 Exclusive	Overlap	0.97	3.33	3.26	3.10	3.45
TIER1_GS Exclusive	Overlap	1.00	3.58	3.00	3.24	3.49
Overlap	Exclusives	0.65	4.22	7.15	3.38	7.24

PRC results
Seeds	Recipients	AUPRC	Z-score for Random Seeds (degree-matched)	Z-score for Random Recipients (degree-matched)	Z-score for Random Seeds (uniform)	Z-score for Random Recipients (uniform)
Binary_EA_as1 Exclusive	TIER1_GS	0.06	79.67	123.82	68.99	17.54
TIER1_GS Exclusive	Binary_EA_as1	0.04	38.90	52.22	48.35	38.59
Binary_EA_as1 Exclusive	TIER1_GS Exclusive	0.04	68.39	68.29	45.72	49.88
TIER1_GS Exclusive	Binary_EA_as1 Exclusive	0.03	29.74	37.32	16.43	30.69
Binary_EA_as1 Exclusive	Overlap	0.04	102.60	125.36	105.81	93.88
TIER1_GS Exclusive	Overlap	0.08	157.79	135.96	308.21	191.56
Overlap	Exclusives	0.08	77.26	109.84	48.79	113.13

Z-scores are computed for the experimental area under ROC or PRC based on distributions of the random areas under these curves
Seeds: Genes where diffusion signal starts FROM)
Recipients: Genes that receive the diffusion signal and that are in the other validated group
Random genes are selected either uniformly or degree matched



Number of genes
**	#Total	# Mapped in the network	Not mapped genes	 
Binary_EA_as1 Exclusive	126	121	DUXB;CHURC1-FNTB;CCDC187;TAF11L13;TNFSF12-TNFSF13
TIER1_GS Exclusive	27	26	MS4A
Overlap	4	4